VIRAL PATHOGEN SAMPLING INSTRUCTIONS
VIRAL PATHOGENS TESTED FOR:
Alfalfa mosaic virus (AMV)
Arabis mosaic virus (ArMV)
Beet curly top virus (BCTV)
C. cryptic virus (CCV)
Cucumber mosaic virus (CMV)
Hop latent viroid (HLVd)
Lettuce chlorosis virus (LCV)
Tobacco mosaic virus (TMV)
Tobacco ringspot virus (TRSV)
Tobacco streak virus (TSV)
Tomato bushy stunt virus (TBSV)
***IMPORTANT: To confirm a plant is truly negative for viral pathogens, GENIE LAB recommends testing several times on a single plant in question (3 to 4 different tests/samples), or 10 times per variety if stock mother plants are maintained in a facility (ex. If a vegetative stock room has five different “ABC” stock plants, then two samples should be taken/tested per plant). We also recommend testing every two to four weeks to ensure plants stay virus negative.
MATERIALS NEEDED: Scissors/pruning shears, 10% bleach solution to soak scissors/shears, clean paper towels, pen, disposable gloves, a cooler with ice (or close fridge access), a refrigerator.
1. For each strain/cultivar you wish to test, sterilize a pair of scissors by soaking in 10% bleach solution for 10 minutes and wipe dry with fresh paper towel (this is to ensure there is no contamination between samples).
2. While the scissors are sterilizing, take the document included in the Genie Lab kit and write down the name of the strain/variety you are testing next to the barcode. Do this twice, one for your records and one to mail back with the kit (do this for each strain/variety you are testing).
3. Put on a fresh pair of disposable gloves (for each strain/variety) and remove a 1-cm root and 1-cm stem/leaf sample from the plant and place on clean paper towel.
4. Using the sterilized scissors, cut a petiole sample from the stem/leaf approximately 1-2 centimeters long (Figure 1; Figure 2A).
5. Combine the root and petiole samples (about 100mg).
6. Open the barcoded Sample Tube from the Genie Lab kit and add the combined root and petiole samples and close the tube tightly, thereby inactivating the sample.
7. Submerge the chopped sample in the solution by inverting the tube 5-10 times to ensure all plant material is coated (Figure 2C).
8. Immediately place the sample into a cooler with ice or directly into a refrigerator.
9. Store the samples in the refrigerator overnight and then place the samples and one barcode document into a bubble mailer and ship immediately to the return address using priority shipping.