VIRAL PATHOGEN SAMPLING INSTRUCTIONS
VIRAL PATHOGENS TESTED FOR:
Alfalfa Mosaic Virus (AMV)
Hop Latent Viroid (HpLVd)
Arabis Mosaic Virus (ArMV)
Cucumber Mosaic Virus (CMV)
Lettuce Chlorosis Virus (LCV)
Tobacco Mosaic Virus (TMV)
Tobacco Ringspot Virus (TRSV)
Tomato Bushy Stunt Virus (TBSV)
***IMPORTANT: To confirm a plant is truly negative for viral pathogens, GENIE LAB recommends testing several times on a single plant in question (3 to 4 different tests/samples), or 10 times per variety if stock mother plants are maintained in a facility (ex. If a vegetative stock room has five different “ABC” stock plants, then two samples should be taken/tested per plant). We also recommend testing every two to four weeks to ensure plants stay virus negative.
MATERIALS NEEDED: Scissors/pruning shears, 10% bleach solution to soak scissors/shears, clean paper towels, pen, disposable gloves, a cooler with ice (or close fridge access), a refrigerator.
1. For each variety you wish to test, sterilize a pair of scissors by soaking in 10% bleach solution for 10 minutes and wipe dry with fresh paper towel (this is to ensure there is no contamination between samples).
2. Take the document included in the Genie Lab kit and write down the name of the variety you are testing next to the barcode (do this for each variety you are testing).
3. Put on a fresh pair of disposable gloves (for each variety) and remove a mature leaf from the lower branches of the plant and place on clean paper towel.
4. Using the sterilized scissors, cut a sample approximately the size of a nickel or quarter from the leaf blade just above the petiole (Figure 1; Figure 2A).
5. Chop the plant sample into as small of fragments as possible (Figure 2B).
6. Open the barcoded Sample Tube from the Genie Lab kit, and add the chopped sample and close the tube tightly..
7. Submerge the chopped sample in the solution by inverting the tube 5-10 times to ensure all plant material is coated, thereby inactivating and preserving the sample (Figure 2C).
8. Immediately place the sample into a cooler with ice or directly into a refrigerator.
9. Store the samples in the refrigerator overnight and then place the samples and barcode document into a bubble mailer and ship immediately to the return address using priority shipping.